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Type:

Biochemistry

31 Questions
GATE→2026→General Biology→Biochemistry
Q1
MCQ[1 Mark]
Which of the following enzyme is involved in the protection of cells from reactive oxygen species?
GATE→2026→General Biology→Biochemistry
Q2
MCQ[1 Mark]
The catalytic efficiency of an enzyme following Michaelis-Menten kinetics is defined by
GATE→2026→General Biology→Biochemistry
Q3
MCQ[1 Mark]
Match the carbohydrates Column I with the correct glycosidic linkages involved in Column II. **Column I** P. Amylose Q. Sucrose R. Amylopectin S. Cellulose **Column II** 1. β(1→4)\beta(1\rightarrow4) 2. α(1→4)\alpha(1\rightarrow4) 3. α1→β2\alpha1\rightarrow\beta2 4. α(1→4)\alpha(1\rightarrow4), α(1→6)\alpha(1\rightarrow6)
GATE→2026→General Biology→Biochemistry
Q4
MCQ[1 Mark]
Which one of the following options represents the products of light reactions of photosynthesis in plants?
GATE→2026→General Biology→Biochemistry
Q5
MSQ[1 Mark]
Which of the following drugs inhibit(s) ATP-ADP translocase?
GATE→2026→General Biology→Biochemistry
Q6
NAT[1 Mark]
Given below is the Gibbs free energy change (ΔG)(\Delta G) in kilo Joules per electron equivalent (kJ e−eq−1)(\text{kJ e}^{-}\text{eq}^{-1}) at pH 7.0, of organic and inorganic half reactions. Acetate synthesis: 18CO2+18HCO3−+H++e−→18CH3COO−+38H2OΔG=27.4 kJ e−eq−1\frac{1}{8}CO_{2}+\frac{1}{8}HCO_{3}^{-}+H^{+}+e^{-}\rightarrow\frac{1}{8}CH_{3}COO^{-}+\frac{3}{8}H_{2}O \quad \Delta G=27.4~\text{kJ e}^{-}\text{eq}^{-1} Reduction reaction: 14O2+H++e−→12H2OΔG=−78.72 kJ e−eq−1\frac{1}{4}O_{2}+H^{+}+e^{-}\rightarrow\frac{1}{2}H_{2}O \quad \Delta G=-78.72~\text{kJ e}^{-}\text{eq}^{-1} The free energy change of acetate oxidation to CO2CO_{2}, H2OH_{2}O and HCO3−HCO_{3}^{-} is kJ e−eq−1\text{kJ e}^{-}\text{eq}^{-1}. (rounded off to two decimal places)
GATE→2026→General Biology→Biochemistry
Q7
MCQ[2 Marks]
Match the biomolecules in Column I with their function given in Column II **Column I** P. Lectins Q. P-glycoprotein R. Digitoxigenin S. Mannose 6-phosphate **Column II** 1. Acts as ATP dependent pump to efflux out small molecules 2. Targets lysosomal enzymes to their destination 3. Specific carbohydrate binding proteins 4. Inhibits Na+−K+\text{Na}^{+}-\text{K}^{+} pump
GATE→2026→General Biology→Biochemistry
Q8
MSQ[2 Marks]
An enzyme-catalyzed reaction is found to have ΔG=−100 kj mol−1\Delta G=-100~\text{kj mol}^{-1}. Which of the following statements about this reaction is/are true?
GATE→2026→General Biology→Biochemistry
Q9
NAT[2 Marks]
The electron equivalent per liter (e−eq L−1)(\text{e}^{-}\text{eq L}^{-1}) of acetate ion solution of concentration 10 g L−110\text{ g L}^{-1} is eeq L−1\text{eeq L}^{-1} (rounded off to one decimal place)
GATE→2025→General Biology→Biochemistry
Q10
MCQ[1 Mark]
Which one of the following coenzymes is utilised by alanine racemase for the conversion of L-Alanine to D-Alanine?
GATE→2025→General Biology→Biochemistry
Q11
MCQ[1 Mark]
Correctly match the following Monosaccharides with their respective Epimers. **Monosaccharide** P. D-mannose Q. D-allose R. D-galactose S. D-talose **Epimer** 1. C-3 epimer of D-glucose 2. C-4 epimer of D-glucose 3. C-4 epimer of D-mannose 4. C-2 epimer of D-glucose 5. C-5 epimer of D-glucose
GATE→2025→General Biology→Biochemistry
Q12
NAT[1 Mark]
Within the Michaelis-Menten framework, the ratio of v0/Vmaxv_{0}/V_{max} when [S]=20×Km[S]=20\times K_{m} is (Round off to two decimal places)
GATE→2025→General Biology→Biochemistry
Q13
MCQ[2 Marks]
Correctly match the Inhibitor with its respective Function in mitochondrial respiration. **Inhibitor** P. FCCP Q. Cyanide R. Oligomycin A S. Butyl malonate **Function** 1. Inhibits cytochrome c oxidase 2. Makes the membrane permeable to protons 3. Blocks mitochondrial uptake of succinate 4. Inhibits ATP synthase
GATE→2025→General Biology→Biochemistry
Q14
MCQ[2 Marks]
An octapeptide composed of these L-amino acids - Lys, Thr, Ser, Met, Arg, Trp, Tyr, Glu - was subjected to analyses with the following outcomes: P. The N-terminal sequencing analysis by Sanger's method yielded 'Ser' at the N-terminus. Q. Chymotrypsin treatment gave a pentapeptide, a 'Tyr' containing dipeptide and a free 'Glu'. R. Cyanogen bromide treatment gave two tetrapeptides. S. Trypsin treatment gave two tripeptides and a dipeptide. Which one of the following is the correct octapeptide sequence?
GATE→2025→General Biology→Biochemistry
Q15
MCQ[2 Marks]
Correctly match the Coenzyme with its respective involvement in a specific Reaction type. **Coenzyme** P. Thiamine pyrophosphate Q. Tetrahydrofolate R. Flavin adenine dinucleotide S. 5'-Deoxyadenosyl cobalamin **Reaction type** 1. Acyl group transfer 2. Transfer of one carbon group 3. Transfer of methyl group 4. Oxidation-reduction 5. Aldehyde transfer
GATE→2025→General Biology→Biochemistry
Q16
MSQ[2 Marks]
Which of the following statement(s) is/are true regarding the effect of the concentration of metabolic intermediates on glycolysis in erythrocytes?
GATE→2025→General Biology→Biochemistry
Q17
MSQ[2 Marks]
Which of the following amino acids contain(s) two chiral carbons?
GATE→2025→General Biology→Biochemistry
Q18
NAT[2 Marks]
For the coupled reactions given below Glucose 6-phosphate + H2O →\rightarrow Glucose + Pi (Reaction 1) ATP + Glucose →\rightarrow ADP + Glucose 6-phosphate (Reaction 2) the standard free energy change of ATP hydrolysis at 25∘C25^{\circ}\text{C} is kJ/mol\text{kJ/mol}. The equilibrium constants for Reaction 1 and Reaction 2 are 360 and 800, respectively; Gas constant R=8.314 J mol−1K−1R=8.314~\text{J mol}^{-1}\text{K}^{-1}. (Round off to two decimal places)
GATE→2024→General Biology→Biochemistry
Q19
MCQ[1 Mark]
2-mercaptoethanol breaks the ____ covalent bond between light and heavy chains of an immunoglobulin molecule.
GATE→2024→General Biology→Biochemistry
Q20
MCQ[1 Mark]
An element that is present in a nucleotide but not in a nucleoside is
GATE→2024→General Biology→Biochemistry
Q21
MCQ[1 Mark]
Krebs (TCA) cycle is ____ pathway.
GATE→2024→General Biology→Biochemistry
Q22
MSQ[2 Marks]
Which of the following statements is/are correct about an uncompetitive inhibitor of an enzyme?
GATE→2024→General Biology→Biochemistry
Q23
NAT[2 Marks]
The free energy change of ATP hydrolysis at 25∘C25^{\circ}\text{C} is −32.2 kJ mol−1-32.2\text{ kJ mol}^{-1}. The free energy change for hydrolysis of α\alpha-glycerophosphate to glycerol is −8.2 kJ mol−1-8.2\text{ kJ mol}^{-1} at 25∘C25^{\circ}\text{C}. Using the above information, the free energy change for the formation of α\alpha-glycerophosphate from glycerol and ATP is kJ mol−1\text{kJ mol}^{-1} (Answer in integer).
GATE→2023→General Biology→Biochemistry
Q24
NAT[2 Marks]
The number of different possible ways of forming five intramolecular disulfide bonds with ten cysteine residues of a protein is
GATE→2023→General Biology→Biochemistry
Q25
NAT[2 Marks]
An enzyme (E) catalyzes the biochemical reaction A→BA\rightarrow B with kcatk_{cat} equal to 500 s−1500\text{ s}^{-1}. If the initial reaction velocity (V0)(V_{0}) is 10 μM.s−110~\mu\text{M.s}^{-1} at the total enzyme concentration [Et][E_{t}] of 30 nM and substrate concentration [A] of 40 μM40~\mu\text{M}, the value of KmK_{m} (in μM\mu\text{M}) is
GATE→2022→General Biology→Biochemistry
Q26
MCQ[1 Mark]
The binding free energy of a ligand to its receptor protein is −11.5 kJ mol−1-11.5~\text{kJ mol}^{-1} at 300 K. What is the value of the equilibrium binding constant? Use R=8.314 J mol−1K−1R=8.314~\text{J mol}^{-1}\text{K}^{-1}
GATE→2022→General Biology→Biochemistry
Q27
MCQ[1 Mark]
Which of the following statements about reversible enzyme inhibitors are CORRECT? P. Uncompetitive inhibitors bind only to the enzyme-substrate complex Q. Non-competitive inhibitors bind only at a different site from the substrate R. Competitive inhibitors bind to the same site as the substrate
GATE→2022→General Biology→Biochemistry
Q28
MCQ[1 Mark]
Terpenoids are made of ____ units.
GATE→2022→General Biology→Biochemistry
Q29
MCQ[2 Marks]
Which of the following are CORRECT about protein structure? P. Secondary structure is formed by a repeating pattern of interactions among the polypeptide backbone atoms Q. Tertiary structure is the three-dimensional arrangement of the polypeptide backbone atoms only R. Quaternary structure refers to an assembly of multiple polypeptide subunits
GATE→2022→General Biology→Biochemistry
Q30
MSQ[2 Marks]
Which of the following is(are) COMMON feature(s) for both aerobic and anaerobic bacterial cultures?
GATE→2022→General Biology→Biochemistry
Q31
MSQ[2 Marks]
Which of the following plot(s) is (are) CORRECT for an enzyme that obeys Michaelis-Menten kinetics, assuming [S]≪Km[S]\ll K_{m}? [S] is the concentration of the substrate, KmK_{m} is the Michaelis constant, and v0v_{0} is the initial reaction velocity.
Figure for Question 31

Microbiology

16 Questions
GATE→2026→General Biology→Microbiology
Q32
MCQ[1 Mark]
In nitrogen fixation, to reduce nitrogen to ammonia, which one of the following shows the correct order of electron flow?
GATE→2026→General Biology→Microbiology
Q33
MCQ[1 Mark]
Which one of the following antibiotics blocks protein chain elongation by preventing the action of peptidyl transferase ?
GATE→2026→General Biology→Microbiology
Q34
NAT[1 Mark]
To determine the viable cell count of a bacterial culture, you have plated 50 μL50~\mu\text{L} of a 100-fold diluted sample of the culture on a nutrient agar plate and obtained 20 colonies after overnight incubation. The viable cell count of the culture is CFU mL−1\text{CFU mL}^{-1}. (answer in integer)
GATE→2025→General Biology→Microbiology
Q35
MCQ[1 Mark]
Koch's postulate was established by Robert Koch while working on a disease caused by
GATE→2025→General Biology→Microbiology
Q36
MCQ[1 Mark]
Corynebacterium diphtheriae causes diphtheria in humans, only when this bacterium is infected by
GATE→2025→General Biology→Microbiology
Q37
MSQ[1 Mark]
Which of the following features is/are used to distinguish Archaea from Bacteria?
GATE→2025→General Biology→Microbiology
Q38
MCQ[2 Marks]
Correctly match the Microorganisms with their respective Nutrition and energy requirement. **Microorganisms** P. Photolithoautotrophs Q. Chemoorganoheterotrophs R. Chemolithoautotrophs S. Photoorganoheterotrophs **Nutrition and energy requirement** 1. Use organic compounds as a source of energy, hydrogen, electron and carbon 2. Use light energy and use CO2CO_{2} as their carbon source 3. Use light energy and use organic compounds as electron donor and carbon source 4. Oxidise reduced-inorganic molecules as energy and electron source but derive carbon from organic sources
GATE→2024→General Biology→Microbiology
Q39
MCQ[2 Marks]
Match the disease (Column I) with its biological vector (Column II). **Column I** P. Chagas disease Q. Trypanosomiasis R. Leishmaniasis S. Yellow Fever **Column II** 1. Tsetse flies 2. Mosquitoes 3. Sandflies 4. Reduviid bugs
GATE→2023→General Biology→Microbiology
Q40
MCQ[1 Mark]
Which one of the following statements is TRUE about leghemoglobin?
GATE→2023→General Biology→Microbiology
Q41
MCQ[1 Mark]
The correct sequence of events during bacteriophage infection of a bacterial cell is
GATE→2023→General Biology→Microbiology
Q42
MCQ[2 Marks]
Match the type of bacteria in Group I with their respective growth properties in Group II. **Group I** P. Halophile Q. Piezophile R. Mesophile S. Xerophile **Group II** 1. Grows optimally between 20∘C20^{\circ}\text{C} and 45∘C45^{\circ}\text{C} 2. Grows best at low water activity 3. Grows at high level of salt 4. Grows optimally at high hydrostatic pressure
GATE→2023→General Biology→Microbiology
Q43
MCQ[2 Marks]
Match the virus in Group I with the type of genome it contains in Group II. **Group I** P. T4 bacteriophage Q. SARS-CoV-2 R. Pseudomonas phage ϕ\phi6 S. ϕ\phiX174 bacteriophage **Group II** 1. dsRNA 2. ssDNA 3. dsDNA 4. ssRNA
GATE→2023→General Biology→Microbiology
Q44
MSQ[2 Marks]
Which of the following statement(s) is (are) TRUE about fluoroquinolone drugs?
GATE→2022→General Biology→Microbiology
Q45
MCQ[1 Mark]
In binomial nomenclature, the name of a bacterial strain is written with the first letter of ____ word(s) being capitalized.
GATE→2022→General Biology→Microbiology
Q46
MCQ[1 Mark]
The type of nucleic acid present in λ\lambda-phage is
GATE→2022→General Biology→Microbiology
Q47
MSQ[2 Marks]
Emerging viruses such as SARS-CoV2 cause epidemics. Which of the following process(es) contribute to the rise of such viruses?

Immunology

8 Questions
GATE→2026→General Biology→Immunology
Q48
MCQ[2 Marks]
Match the immunological terms in Column I with their function/description in the Column II **Column I** P. J gene Q. Cyclosporin R. Epitope S. Hapten **Column II** 1. Suppresses immune system by blocking calcineurin 2. Antibody binding site on a large molecule 3. Small foreign molecule which elicits an immune response only when combined with a carrier molecule 4. Contributes to antibody diversity
GATE→2025→General Biology→Immunology
Q49
MCQ[1 Mark]
The diversity in T-cell receptors is generated by
GATE→2025→General Biology→Immunology
Q50
MCQ[2 Marks]
Correctly match the type of Hypersensitivity reaction with its respective Example. **Hypersensitivity reaction** P. Type I Q. Type II R. Type III S. Type IV **Example** 1. Tuberculin reaction 2. Arthus reaction 3. Chronic urticaria 4. Systemic anaphylaxis
GATE→2023→General Biology→Immunology
Q51
MCQ[1 Mark]
Which one of the following drugs is NOT an immune checkpoint inhibitor?
GATE→2023→General Biology→Immunology
Q52
MCQ[1 Mark]
Dendritic cells are involved in cross-presentation of antigens. Which of the following protein(s) is (are) required for cross-presentation? P. Basic leucine zipper ATF-like transcription factor 3 (BATF3) Q. Membrane associated ring-CH-type finger 1 (MARCH-1) R. Solute carrier family 10 member 1 (SLC10A1) S. Class II-associated invariant chain peptide (CLIP)
GATE→2023→General Biology→Immunology
Q53
MCQ[1 Mark]
Which one of the following is required for the development of B-cells in the bone marrow?
GATE→2023→General Biology→Immunology
Q54
MCQ[2 Marks]
Match the immune tolerance mechanisms in Group I with their respective outcomes in Group II. **Group I** P. Anergy Q. Activation-induced cell death R. Receptor editing S. Regulatory T-cells **Group II** 1. Elimination of activated T-cells after antigen clearance 2. Inhibition of auto-reactive T-cells at periphery 3. Unresponsiveness to antigens due to lack of co-stimulatory molecules 4. Elimination of auto-reactive B-cells
GATE→2022→General Biology→Immunology
Q55
MCQ[2 Marks]
Match the cell type (Column I) with its function (Column II). **Column I** P. B cells Q. Neutrophils R. T cells S. Mast cells **Column II** 1. Humoral immunity 2. Cytotoxicity 3. Histamine-associated allergy 4. Phagocytosis